matlab 9.9 2020b Search Results


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matlab bioinformatics toolbox  (MathWorks Inc)
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mkate2̂ seĉ 3xmyc dickinson  (Addgene inc)
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Figure 3. Dynein and LIN-5/NuMA are required for germ cell spindle orientation and germline tissue organization (A) Confocal sections through the mid-plane of gonad arms from L4 larvae expressing DHC-1::AID::GFP (dynein, left) or LIN-5::mAID::mNG (NuMA, right), with a germline-specific TIR1 (sun-1p::TIR1), after a 40-min auxin treatment, as compared to untreated animals. The germ line is outlined in white. Scale bar, 10 mm (B) Quantification of the fluorescence intensity of each FP-tagged protein in the germ line. Measurements were normalized to the mean value for non-depleted germ lines. (C) Cumulative distribution of spindle angles at anaphase to the gonad surface normal vector, comparing control animals to those depleted of DHC-1/dynein or LIN-5/NuMA as in (A) and (B). Depletion of either DHC-1/dynein or LIN-5/NuMA reduces the spindle orientation bias to the gonad surface. The theoretical random distribution (yellow) is shown for reference. (D) Top: maximum-intensity projections of gonad arms from L4 larvae expressing mKate2::ANI-1, depleted (right) or not (left) of LIN-5/NuMA in the germ line by a 6-h auxin treatment. Bottom: reconstruction of the distal rachis surface using the mKate2::ANI-1 signal showing one rendered object in the non-depleted germ line (gray) and two objects in the depleted germ line (gray and yellow). Scale bars, 10 mm (E) Distal rachis volume in control and LIN-5/NuMA-depleted animals. Each dot represents the rachis for one gonad arm. Bars represent the mean ± standard deviation. (F) The number of rendered rachis objects in control and LIN-5/NuMA-depleted animals. For all panels, *p < 0.05, **p < 0.01, and ***p < 0.001. Summary statistics and statistical tests used are given in Table S3. See also Figure S3.
Mkate2̂ Seĉ 3xmyc Dickinson, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sjj c38c10 4 maid mclover hygro natsume  (Addgene inc)
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Figure 3. Dynein and LIN-5/NuMA are required for germ cell spindle orientation and germline tissue organization (A) Confocal sections through the mid-plane of gonad arms from L4 larvae expressing DHC-1::AID::GFP (dynein, left) or LIN-5::mAID::mNG (NuMA, right), with a germline-specific TIR1 (sun-1p::TIR1), after a 40-min auxin treatment, as compared to untreated animals. The germ line is outlined in white. Scale bar, 10 mm (B) Quantification of the fluorescence intensity of each FP-tagged protein in the germ line. Measurements were normalized to the mean value for non-depleted germ lines. (C) Cumulative distribution of spindle angles at anaphase to the gonad surface normal vector, comparing control animals to those depleted of DHC-1/dynein or LIN-5/NuMA as in (A) and (B). Depletion of either DHC-1/dynein or LIN-5/NuMA reduces the spindle orientation bias to the gonad surface. The theoretical random distribution (yellow) is shown for reference. (D) Top: maximum-intensity projections of gonad arms from L4 larvae expressing mKate2::ANI-1, depleted (right) or not (left) of LIN-5/NuMA in the germ line by a 6-h auxin treatment. Bottom: reconstruction of the distal rachis surface using the mKate2::ANI-1 signal showing one rendered object in the non-depleted germ line (gray) and two objects in the depleted germ line (gray and yellow). Scale bars, 10 mm (E) Distal rachis volume in control and LIN-5/NuMA-depleted animals. Each dot represents the rachis for one gonad arm. Bars represent the mean ± standard deviation. (F) The number of rendered rachis objects in control and LIN-5/NuMA-depleted animals. For all panels, *p < 0.05, **p < 0.01, and ***p < 0.001. Summary statistics and statistical tests used are given in Table S3. See also Figure S3.
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fire90 rrid addgene 1654 gpr 1 2 feeding rnai kamath  (Addgene inc)
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Figure 3. Dynein and LIN-5/NuMA are required for germ cell spindle orientation and germline tissue organization (A) Confocal sections through the mid-plane of gonad arms from L4 larvae expressing DHC-1::AID::GFP (dynein, left) or LIN-5::mAID::mNG (NuMA, right), with a germline-specific TIR1 (sun-1p::TIR1), after a 40-min auxin treatment, as compared to untreated animals. The germ line is outlined in white. Scale bar, 10 mm (B) Quantification of the fluorescence intensity of each FP-tagged protein in the germ line. Measurements were normalized to the mean value for non-depleted germ lines. (C) Cumulative distribution of spindle angles at anaphase to the gonad surface normal vector, comparing control animals to those depleted of DHC-1/dynein or LIN-5/NuMA as in (A) and (B). Depletion of either DHC-1/dynein or LIN-5/NuMA reduces the spindle orientation bias to the gonad surface. The theoretical random distribution (yellow) is shown for reference. (D) Top: maximum-intensity projections of gonad arms from L4 larvae expressing mKate2::ANI-1, depleted (right) or not (left) of LIN-5/NuMA in the germ line by a 6-h auxin treatment. Bottom: reconstruction of the distal rachis surface using the mKate2::ANI-1 signal showing one rendered object in the non-depleted germ line (gray) and two objects in the depleted germ line (gray and yellow). Scale bars, 10 mm (E) Distal rachis volume in control and LIN-5/NuMA-depleted animals. Each dot represents the rachis for one gonad arm. Bars represent the mean ± standard deviation. (F) The number of rendered rachis objects in control and LIN-5/NuMA-depleted animals. For all panels, *p < 0.05, **p < 0.01, and ***p < 0.001. Summary statistics and statistical tests used are given in Table S3. See also Figure S3.
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Image Search Results


Figure 3. Dynein and LIN-5/NuMA are required for germ cell spindle orientation and germline tissue organization (A) Confocal sections through the mid-plane of gonad arms from L4 larvae expressing DHC-1::AID::GFP (dynein, left) or LIN-5::mAID::mNG (NuMA, right), with a germline-specific TIR1 (sun-1p::TIR1), after a 40-min auxin treatment, as compared to untreated animals. The germ line is outlined in white. Scale bar, 10 mm (B) Quantification of the fluorescence intensity of each FP-tagged protein in the germ line. Measurements were normalized to the mean value for non-depleted germ lines. (C) Cumulative distribution of spindle angles at anaphase to the gonad surface normal vector, comparing control animals to those depleted of DHC-1/dynein or LIN-5/NuMA as in (A) and (B). Depletion of either DHC-1/dynein or LIN-5/NuMA reduces the spindle orientation bias to the gonad surface. The theoretical random distribution (yellow) is shown for reference. (D) Top: maximum-intensity projections of gonad arms from L4 larvae expressing mKate2::ANI-1, depleted (right) or not (left) of LIN-5/NuMA in the germ line by a 6-h auxin treatment. Bottom: reconstruction of the distal rachis surface using the mKate2::ANI-1 signal showing one rendered object in the non-depleted germ line (gray) and two objects in the depleted germ line (gray and yellow). Scale bars, 10 mm (E) Distal rachis volume in control and LIN-5/NuMA-depleted animals. Each dot represents the rachis for one gonad arm. Bars represent the mean ± standard deviation. (F) The number of rendered rachis objects in control and LIN-5/NuMA-depleted animals. For all panels, *p < 0.05, **p < 0.01, and ***p < 0.001. Summary statistics and statistical tests used are given in Table S3. See also Figure S3.

Journal: Cell reports

Article Title: The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line.

doi: 10.1016/j.celrep.2025.115296

Figure Lengend Snippet: Figure 3. Dynein and LIN-5/NuMA are required for germ cell spindle orientation and germline tissue organization (A) Confocal sections through the mid-plane of gonad arms from L4 larvae expressing DHC-1::AID::GFP (dynein, left) or LIN-5::mAID::mNG (NuMA, right), with a germline-specific TIR1 (sun-1p::TIR1), after a 40-min auxin treatment, as compared to untreated animals. The germ line is outlined in white. Scale bar, 10 mm (B) Quantification of the fluorescence intensity of each FP-tagged protein in the germ line. Measurements were normalized to the mean value for non-depleted germ lines. (C) Cumulative distribution of spindle angles at anaphase to the gonad surface normal vector, comparing control animals to those depleted of DHC-1/dynein or LIN-5/NuMA as in (A) and (B). Depletion of either DHC-1/dynein or LIN-5/NuMA reduces the spindle orientation bias to the gonad surface. The theoretical random distribution (yellow) is shown for reference. (D) Top: maximum-intensity projections of gonad arms from L4 larvae expressing mKate2::ANI-1, depleted (right) or not (left) of LIN-5/NuMA in the germ line by a 6-h auxin treatment. Bottom: reconstruction of the distal rachis surface using the mKate2::ANI-1 signal showing one rendered object in the non-depleted germ line (gray) and two objects in the depleted germ line (gray and yellow). Scale bars, 10 mm (E) Distal rachis volume in control and LIN-5/NuMA-depleted animals. Each dot represents the rachis for one gonad arm. Bars represent the mean ± standard deviation. (F) The number of rendered rachis objects in control and LIN-5/NuMA-depleted animals. For all panels, *p < 0.05, **p < 0.01, and ***p < 0.001. Summary statistics and statistical tests used are given in Table S3. See also Figure S3.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER C. elegans: Strain HS3911; genotype: lin-5(os205[lin-5::mAID::mNG] II This study HS3911 C. elegans: Strain PD1594; genotype: ccTi1594 [mex-5p::GFP::gpr-1::smu-1 30UTR + Cbr-unc-119(+), III: 680195] III CGC PD1594 Oligonucleotides Guide RNA sequence for generating LIN5:mAID:mNG: 50-GTCCAAGAAAAAGAA CCGTC-30 Heppert et al.86 N/A Primers for generating LIN-5:mAID:mNG, see Table S1 This study N/A Recombinant DNA L4440 (RNAi empty vector for feeding) Timmons and Fire90 RRID: Addgene_1654 gpr-1/2 feeding RNAi Kamath et al.79 sjj_C38C10.4 mAID-mClover-Hygro Natsume et al.91 RRID: Addgene_72828 mNG-mom-5 Heppert et al.86 mKate2̂ SEĈ 3xMyc Dickinson et al.92 RRID: Addgene_70685 lin-5:mAID:mNG This study pTN26 Software and algorithms Fiji 1.52v Schindelin et al.93 Fiji (RRID:SCR_002285) MATLAB 2020b MathWorks94 MATLAB (RRID:SCR_001622) Trackmate 6.0.0 Tinevez et al.95 https://imagej.net/plugins/trackmate/ Imaris 9.2.1 Oxford Instruments96 Imaris (RRID:SCR_007370) Julia 1.10.4 Bezanson et al.97 Julia Programming Language (RRID:SCR_021666) Python 3 Van Rossum and Drake98 Python Programming Language (RRID:SCR_008394) Custom scripts This study https://github.com/VincentPoupart/ Zellag2024 CentTracker scripts Zellag et al.37 https://github.com/yifnzhao/CentTracker Other Microfabricated silica wafer Zellag et al.37,38; Gerhold et al.99 N/A Glass slides with wells for gonad explant culture and imaging Fisher Scientific 30-2066A-BROWN 3 SQUARE 14mm with Bars Epoxy autoclavable Cell Reports 44, 115296, February 25, 2025 17

Techniques: Expressing, Plasmid Preparation, Control, Standard Deviation